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human melanocyte basal medium kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher human melanocyte basal medium kit
    A . Graphical illustration of differentiating from embryonic stem cells to <t>melanocytes</t> B . Pigmented and dendritic cells appeared 7-10 days after exposure to puromycin at passage 0 (p0). C . Pigmented cells increased, while non-pigmented cells decreased around passage 9 (p9). D . Pigmented cells were dominant by passage 15. E . Gross appearance of pigmented cells. The culture plate was covered with black colonies (left), and the cell pellet was black without any staining (right). F-G . <t>Human</t> Embryonic stem cells-derived melanocytes (F: EMCs) at passage 25 resembled tissue-separated melanocytes (G: TMCs) in morphology. H . Quantitative RT-PCR analysis of the genes for NANOG, OCT4, TYRP1, MITF, Tyrosinase, PAX3, <t>Kit,</t> and DCT, in undifferentiated cells (UDCs), EMCs, and TMCs. The expression levels were normalized by expressions of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The expression levels of TMC were set at 1.0. Each expression level was calculated from the results of triplicate technical experiments and the charts are drawn as the average ± standard deviation. I . Immunocytochemical analysis of EMCs(left) and TMC (right) with antibodies against MITF (red), Tyrosinase (red), and MelanA (red). Nuclei were counterstained with DAPI (blue).
    Human Melanocyte Basal Medium Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+melanocyte+basal+medium+kit/bio_rxiv__2023__03__03__530736-37-10-15?v=Thermo+Fisher
    Average 90 stars, based on 1 article reviews
    human melanocyte basal medium kit - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Functional and long-lived melanocytes from human pluripotent stem cells with transient ectopic expression of JMJD3"

    Article Title: Functional and long-lived melanocytes from human pluripotent stem cells with transient ectopic expression of JMJD3

    Journal: bioRxiv

    doi: 10.1101/2023.03.03.530736

    A . Graphical illustration of differentiating from embryonic stem cells to melanocytes B . Pigmented and dendritic cells appeared 7-10 days after exposure to puromycin at passage 0 (p0). C . Pigmented cells increased, while non-pigmented cells decreased around passage 9 (p9). D . Pigmented cells were dominant by passage 15. E . Gross appearance of pigmented cells. The culture plate was covered with black colonies (left), and the cell pellet was black without any staining (right). F-G . Human Embryonic stem cells-derived melanocytes (F: EMCs) at passage 25 resembled tissue-separated melanocytes (G: TMCs) in morphology. H . Quantitative RT-PCR analysis of the genes for NANOG, OCT4, TYRP1, MITF, Tyrosinase, PAX3, Kit, and DCT, in undifferentiated cells (UDCs), EMCs, and TMCs. The expression levels were normalized by expressions of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The expression levels of TMC were set at 1.0. Each expression level was calculated from the results of triplicate technical experiments and the charts are drawn as the average ± standard deviation. I . Immunocytochemical analysis of EMCs(left) and TMC (right) with antibodies against MITF (red), Tyrosinase (red), and MelanA (red). Nuclei were counterstained with DAPI (blue).
    Figure Legend Snippet: A . Graphical illustration of differentiating from embryonic stem cells to melanocytes B . Pigmented and dendritic cells appeared 7-10 days after exposure to puromycin at passage 0 (p0). C . Pigmented cells increased, while non-pigmented cells decreased around passage 9 (p9). D . Pigmented cells were dominant by passage 15. E . Gross appearance of pigmented cells. The culture plate was covered with black colonies (left), and the cell pellet was black without any staining (right). F-G . Human Embryonic stem cells-derived melanocytes (F: EMCs) at passage 25 resembled tissue-separated melanocytes (G: TMCs) in morphology. H . Quantitative RT-PCR analysis of the genes for NANOG, OCT4, TYRP1, MITF, Tyrosinase, PAX3, Kit, and DCT, in undifferentiated cells (UDCs), EMCs, and TMCs. The expression levels were normalized by expressions of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The expression levels of TMC were set at 1.0. Each expression level was calculated from the results of triplicate technical experiments and the charts are drawn as the average ± standard deviation. I . Immunocytochemical analysis of EMCs(left) and TMC (right) with antibodies against MITF (red), Tyrosinase (red), and MelanA (red). Nuclei were counterstained with DAPI (blue).

    Techniques Used: Staining, Derivative Assay, Quantitative RT-PCR, Expressing, Standard Deviation



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    human melanocyte basal medium kit  (Thermo Fisher)
    90
    Thermo Fisher human melanocyte basal medium kit
    A . Graphical illustration of differentiating from embryonic stem cells to <t>melanocytes</t> B . Pigmented and dendritic cells appeared 7-10 days after exposure to puromycin at passage 0 (p0). C . Pigmented cells increased, while non-pigmented cells decreased around passage 9 (p9). D . Pigmented cells were dominant by passage 15. E . Gross appearance of pigmented cells. The culture plate was covered with black colonies (left), and the cell pellet was black without any staining (right). F-G . <t>Human</t> Embryonic stem cells-derived melanocytes (F: EMCs) at passage 25 resembled tissue-separated melanocytes (G: TMCs) in morphology. H . Quantitative RT-PCR analysis of the genes for NANOG, OCT4, TYRP1, MITF, Tyrosinase, PAX3, <t>Kit,</t> and DCT, in undifferentiated cells (UDCs), EMCs, and TMCs. The expression levels were normalized by expressions of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The expression levels of TMC were set at 1.0. Each expression level was calculated from the results of triplicate technical experiments and the charts are drawn as the average ± standard deviation. I . Immunocytochemical analysis of EMCs(left) and TMC (right) with antibodies against MITF (red), Tyrosinase (red), and MelanA (red). Nuclei were counterstained with DAPI (blue).
    Human Melanocyte Basal Medium Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+melanocyte+basal+medium+kit/bio_rxiv__2023__03__03__530736-37-10-15?v=Thermo+Fisher
    Average 90 stars, based on 1 article reviews
    human melanocyte basal medium kit - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A . Graphical illustration of differentiating from embryonic stem cells to melanocytes B . Pigmented and dendritic cells appeared 7-10 days after exposure to puromycin at passage 0 (p0). C . Pigmented cells increased, while non-pigmented cells decreased around passage 9 (p9). D . Pigmented cells were dominant by passage 15. E . Gross appearance of pigmented cells. The culture plate was covered with black colonies (left), and the cell pellet was black without any staining (right). F-G . Human Embryonic stem cells-derived melanocytes (F: EMCs) at passage 25 resembled tissue-separated melanocytes (G: TMCs) in morphology. H . Quantitative RT-PCR analysis of the genes for NANOG, OCT4, TYRP1, MITF, Tyrosinase, PAX3, Kit, and DCT, in undifferentiated cells (UDCs), EMCs, and TMCs. The expression levels were normalized by expressions of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The expression levels of TMC were set at 1.0. Each expression level was calculated from the results of triplicate technical experiments and the charts are drawn as the average ± standard deviation. I . Immunocytochemical analysis of EMCs(left) and TMC (right) with antibodies against MITF (red), Tyrosinase (red), and MelanA (red). Nuclei were counterstained with DAPI (blue).

    Journal: bioRxiv

    Article Title: Functional and long-lived melanocytes from human pluripotent stem cells with transient ectopic expression of JMJD3

    doi: 10.1101/2023.03.03.530736

    Figure Lengend Snippet: A . Graphical illustration of differentiating from embryonic stem cells to melanocytes B . Pigmented and dendritic cells appeared 7-10 days after exposure to puromycin at passage 0 (p0). C . Pigmented cells increased, while non-pigmented cells decreased around passage 9 (p9). D . Pigmented cells were dominant by passage 15. E . Gross appearance of pigmented cells. The culture plate was covered with black colonies (left), and the cell pellet was black without any staining (right). F-G . Human Embryonic stem cells-derived melanocytes (F: EMCs) at passage 25 resembled tissue-separated melanocytes (G: TMCs) in morphology. H . Quantitative RT-PCR analysis of the genes for NANOG, OCT4, TYRP1, MITF, Tyrosinase, PAX3, Kit, and DCT, in undifferentiated cells (UDCs), EMCs, and TMCs. The expression levels were normalized by expressions of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The expression levels of TMC were set at 1.0. Each expression level was calculated from the results of triplicate technical experiments and the charts are drawn as the average ± standard deviation. I . Immunocytochemical analysis of EMCs(left) and TMC (right) with antibodies against MITF (red), Tyrosinase (red), and MelanA (red). Nuclei were counterstained with DAPI (blue).

    Article Snippet: The medium was ESTEM-HE medium during purification and changed to Human Melanocyte Basal Medium Kit (Gibco) or Melanocyte Growth Basal Medium-4 (Lonza, Switzerland) after pigmented cells were dominant.

    Techniques: Staining, Derivative Assay, Quantitative RT-PCR, Expressing, Standard Deviation